Inherited Genomics
What is the 3D Tumor Spheroid Assay?
The 3D Tumor Spheroid Assay is a crucial tool used for analyzing the therapeutic effect and tumor-penetrating capacity of anti-cancer therapeutics. This assay is performed on spheroids with high translational value, making it ideal for lead candidate selection for in vivo studies. Unlike 2D models, 3D spheroids recapitulate complex in vivo conditions, including cellular barriers and gradients of oxygen and nutrients, thus providing a higher predictive value for pre-clinical drug research.
Why Use the 3D Tumor Spheroid Assay?
Realistic Tumor Simulation: The 3D spheroid model mimics in vivo environments with various cellular states and metabolic activity.
High Predictive Value: Suitable for testing both anti-proliferative and cytotoxic drug effects.
Effective Lead Candidate Selection: Facilitates the evaluation of compounds for further in vivo studies.
Applications and Methodologies
3D Spheroid Formation Assay: Tumor cells form spheroids in ultra-low attachment multiwell plates, growing to a significant size within 6 days.
High-Throughput Compatibility: Allows for efficient large-scale testing.
Mono-Spheroid and Co-Culture Spheroid Testing: Offers testing with human tumor cell lines and quantification of both stroma and tumor cells.
Explore More: Soft Agar Assay
In addition to the 3D Tumor Spheroid Assay, we offer the Soft Agar Assay for comprehensive cancer research. This 3D assay is used to test the proliferation of single cells into colonies, providing valuable insights into a drug's impact on cellular growth and spread.
Comparison of 3D Assays
3D Tumor Spheroid Assay: Focuses on analyzing existing spheroid responses to therapeutics.
Soft Agar Assay: Ideal for studying single-cell proliferation and colony formation.
How it works
Our step-by-step approach
With concepts in hand, we meticulously design, refining every detail to align with your vision and objectives.